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Cryo EM Sample Preparation

The imaging capabilities of the facility are supported by a complete pipeline of state-of-the-art sample preparation equipment for cryo applications.

Cryo samples are vitrified by automated plunge freezing (FEI Vitrobot) or by high pressure freezing (Baltec HPM010 or freezing after light-stimulation, Leica HPM100). Cryo-ultramicrotomy (also of vitreous samples) is done in an area of controlled temperature and humidity at a Leica FC6 ultramicrotome. On the light microscopy site, sample preparation and correlative work is supported by a Zeiss LSM 700 confocal microscope which has a cryostage attached to enable visualization of vitrified samples by fluorescent light.


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