General EM Sample Preparation
Various general EM sample preparation equipment is available. Room Temperature Microtomy is done at a Leica UC7 ultramicrotome or a Reichert Ultracut S.
Identification of fluorescent structures down to 1.1 micron immediately before high pressure freezing is done at a fully apochromatic corrected stereo microscope with 16.5:1 zoom optics (Leica M165 FC stereo microscope). Carbon films of high quality and reproducible thickness are generated by a Cressington Carbon Coater type 208.